V Ferrara, A Ottaviani, F Cavaleri, G Arrabito, P Cancemi, YP Ho, BR Knudsen, MS Hede, C Pellerito, A Desideri, S Feo, G Marletta, B Pignataro: DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection. In: 539 , pp. 309-316, 2019.

Abstract

Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.

    BibTeX (Download)

    @article{Ferrara2018,
    title = {DNA-based biosensor on flexible nylon substrate by dip-pen lithography for topoisomerase detection},
    author = {V Ferrara and A Ottaviani and F Cavaleri and G Arrabito and P Cancemi and YP Ho and BR Knudsen and MS Hede and C Pellerito and A Desideri and S Feo and G Marletta and B Pignataro},
    year  = {2019},
    date = {2019-01-18},
    booktitle = {Lecture Notes in Electrical Engineering},
    volume = {539},
    pages = {309-316},
    organization = {4th National Conference on Sensors},
    abstract = {Dip-pen lithography (DPL) technique has been employed to develop a new flexible biosensor realized on nylon with the aim to detect the activity of human topoisomerase. The sensor is constituted by an ordered array of a DNA substrate on flexible nylon supports that can be exploited as a drug screening platform for anticancer molecules. Here, we demonstrate a rapid protocol that permits to immobilize minute quantities of DNA oligonucleotides by DPL on nylon surfaces. Theoretical and experimental aspects have been investigated to successfully print DNA oligonucleotides by DPL on such a porous and irregular substrate.},
    keywords = {A. Desideri},
    pubstate = {published},
    tppubtype = {article}
    }